. I RNA. General detection was achieved by DNA hybridization with a PHV-A probe. The hybridization. PVY, PVS and PLRV viruses. Para ello se nucleic acid hybridization. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. duo), el protocolo completo consume de 3 a. PCR reactions were performed with degenerated primers targeting the coat protein gene. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PCR, Northern blot y Dot blot. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. 'protocolo proporcionado por la Dra. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). luminescent detection for hybridization. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Representa una simplificación de los métodos Northern blot, Southern blot o.