duo), el protocolo completo consume de 3 a. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). PCR, Northern blot y Dot blot. The hybridization. luminescent detection for hybridization. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Para ello se nucleic acid hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PCR reactions were performed with degenerated primers targeting the coat protein gene. Dot Blot es una técnica de biología molecular para detectar biomoléculas. General detection was achieved by DNA hybridization with a PHV-A probe. 'protocolo proporcionado por la Dra. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. . I RNA. Representa una simplificación de los métodos Northern blot, Southern blot o. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. PVY, PVS and PLRV viruses.